S5 Fig. S9 Fig. PLoS Biol 15 12 : e Electron microscopy and single particle analysis Electron microscopy was performed on freshly prepared complexes same day of purification. To obtain information about the overall structure of the light-harvesting systems of G. In contrast, CD spectra of isolated antenna complexes LH1, LH2, B typically exhibit large, often nearly conservative carotenoid bands [ 1921 — 23 ]. Here, we analyzed photosynthetic complexes in Gemmatimonas phototrophicathe only known phototrophic representative of the bacterial phylum Gemmatimonadetes.
The 2 most intense bands, with apparent molecular masses approximately 5 kDa, most likely originated from light-harvesting antenna subunits S3B Fig. Femtosecond energy-transfer processes in the B light-harvesting complex of Rhodobacter sphaeroides 2. This means that the PS complex of G. This predicted organization with 2 concentric rings composed of 15 dimers each harboring 2 BChl a molecules translates in total to 60 BChl a molecules, which is consistent with the number of BChl a determined by the liquid chromatography. During chromatography, the majority of carotenoids eluted differently from the PS complexes S2 Fig. Biophys J. The carotenoids exhibited very low LD. Konstitucijos pr.
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This pattern is well-known from systems containing excitonically coupled BChls, such as LH1 [ 32 ] or LH2 [ 33 ] complexes of purple bacteria. A gradually increasing delay between the 2 pulses was set by a computer-controlled delay line in the probe pathway. A femtosecond transient absorption study. Isolation and characterization of a structural subunit from the core light-harvesting complex of Rhodobacter sphaeroides 2. Example of a raw TEM image of G. The data were derived from 23 measurements from 4 individual complex images. The complex contains Light-harvesting complexes in G.
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